INDUKSI EMBRIO SOMATIK DARI TANAMAN KAKAO ADAPTIVE ACEH MENGGUNAKAN EKSPLAN BUNGA SERTA ZAT PENGATUR TUMBUH PICLORAM

Induction of Embryo Somatic From Cacao Adaptive Aceh Using Flower Eksplant with Plant Growth Regulator Picloram

Zuyasna dan Siti Hafsah

Program Studi Agroteknologi Fakulas Pertanian, Universitas Syiah Kuala, Banda Aceh. Email penulis pertama: zuyasna@yahoo.com

 

ABSTRACT

In order to fulfill the cocoa revitalization program, relatively large quantities of seedling are needed. Tissue culture is one of the alternative techniques for vegetative propagation that produce the large numbers of seedlings and uniform in a relatively short time, and also does not depend on the season. A preliminary study to induce callus and embryo somatic cocoa clones adaptive in Aceh has been carried out using immature flower parts of cocoa. The result showed that picloram was able to produce somatic embryos of staminode of various explants. Callus growth began to appear after two weeks on staminode, and then were subcultured into the same medium to produce secondary somatic embryos.

 

Keywords: picloram, BAP, cacao, callus, somatic embryo

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